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2016 WHO Classification of Acute Leukemias of Ambiguous Lineage For acute leukemias of ambiguous lineage, the group of acute leukemias that have characteristics of both acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL), the WHO classification system is summarized in Table 1.[2,3] The criteria for lineage assignment for a diagnosis of mixed phenotype acute leukemia (MPAL) are provided in Table 2.[4] Leukemias of mixed phenotype may be seen in various presentations, including the following: Bilineal leukemias in which there are two distinct populations of cells, usually one lymphoid and one myeloid

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Eighteen hours later, the culture medium of adherent cells was replaced with sterile Seahorse XF base media (Cat# 102353-100 for OCR and Cat# 103335-100 for ECAR) supplemented with 10 mM D-glucose, 2 mM L-glutamine, and 1 mM sodium PA (pH 7.4)